Screening of genomic libraries.

Valdenice M Novelli, Mariângela Cristofani-Yaly, Marinês Bastianel, Dario A Palmieri, Marcos A Machado

Instituto Agronômico, Centro APTA Citros Sylvio Moreira, Cordeirópolis, SP, Brazil.

Methods in molecular biology (Clifton, N.J.) 2013

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Microsatellites, or simple sequence repeats (SSRs), have proven to be an important molecular marker in plant genetics and breeding research. The main strategies to obtain these markers can be through genomic DNA and from expressed sequence tags (ESTs) from mRNA/cDNA libraries. Genetic studies using microsatellite markers have increased rapidly because they can be highly polymorphic, codominant markers and they show heterozygous conserved sequences. Here, we describe a methodology to obtain microsatellite using the enrichment library of DNA genomic sequences. This method is highly efficient to development microsatellite markers especially in plants that do not have available ESTs or genome databases. This methodology has been used to enrich SSR marker libraries in Citrus spp., an important tool to genotype germplasm, to select zygotic hybrids, and to saturate genetic maps in breeding programs.

Citation

Valdenice M Novelli, Mariângela Cristofani-Yaly, Marinês Bastianel, Dario A Palmieri, Marcos A Machado. Screening of genomic libraries. Methods in molecular biology (Clifton, N.J.). 2013;1006:17-24