Agarose gel electrophoresis and polyacrylamide gel electrophoresis for visualization of simple sequence repeats.

James Anderson, Drew Wright, Khalid Meksem

Department of Plant, Soil, and General Agriculture, Southern Illinois University at Carbondale, Carbondale, IL, USA.

Methods in molecular biology (Clifton, N.J.) 2013

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In the modern age of genetic research there is a constant search for ways to improve the efficiency of plant selection. The most recent technology that can result in a highly efficient means of selection and still be done at a low cost is through plant selection directed by simple sequence repeats (SSRs or microsatellites). The molecular markers are used to select for certain desirable plant traits without relying on ambiguous phenotypic data. The best way to detect these is the use of gel electrophoresis. Gel electrophoresis is a common technique in laboratory settings which is used to separate deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) by size. Loading DNA and RNA onto gels allows for visualization of the size of fragments through the separation of DNA and RNA fragments. This is achieved through the use of the charge in the particles. As the fragments separate, they form into distinct bands at set sizes. We describe the ability to visualize SSRs on slab gels of agarose and polyacrylamide gel electrophoresis.

Citation

James Anderson, Drew Wright, Khalid Meksem. Agarose gel electrophoresis and polyacrylamide gel electrophoresis for visualization of simple sequence repeats. Methods in molecular biology (Clifton, N.J.). 2013;1006:167-77