Microsatellite fragment analysis using the ABI PRISM ® 377 DNA sequencer.

Mark A Renshaw, Melissa Giresi, J Orville Adams

Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA.

Methods in molecular biology (Clifton, N.J.) 2013

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The ABI PRISM (®) 377 DNA Sequencer is used for a variety of microsatellite-based research. The platform provides researchers with a cost-effective means for high-throughput genotyping, which can be further optimized by multiplexing microsatellite loci or by using a tail-labeling approach to screen large sets of markers. The goals of this chapter are to present a protocol for performing microsatellite-based analyses on the ABI 377 and to provide researchers with information on how to troubleshoot common issues associated with running the ABI 377 sequencers.

Citation

Mark A Renshaw, Melissa Giresi, J Orville Adams. Microsatellite fragment analysis using the ABI PRISM ® 377 DNA sequencer. Methods in molecular biology (Clifton, N.J.). 2013;1006:181-96