Neuropeptide Y in black seabream Acanthopagrus schlegelii: identification, distribution and mRNA expression responses to ghrelin.

X L Ma, L W Zheng, L T Mao, L B Zhou, A L Wang

Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, 55 Zhongshan West Road, Guangzhou, 510631, PR China.

Journal of fish biology 2013 Apr

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The coding region of neuropeptide Y (NPY) complementary (c)DNA was cloned from the hypothalamus RNA of black seabream Acanthopagrus schlegelii, including 297 bp coding for prepro-NPY of 98 amino acids. Real-time reverse transcriptase-polymerase chain reaction was performed to determine A. schlegelii npy gene expression; NPY messenger RNA (mRNA) was expressed highly in the brain and stomach. Treatment with acylated ghrelin significantly up-regulated NPY mRNA level in the hypothalamus, suggesting that NPY may be involved in regulating food intake of A. schlegelii. © 2013 The Authors. Journal of Fish Biology © 2013 The Fisheries Society of the British Isles.

Citation

X L Ma, L W Zheng, L T Mao, L B Zhou, A L Wang. Neuropeptide Y in black seabream Acanthopagrus schlegelii: identification, distribution and mRNA expression responses to ghrelin. Journal of fish biology. 2013 Apr;82(4):1441-7