Development of an anti-norovirus single-chain Fv for immunochromatographic test kit.
Michihiko Nakano, Keita Murofushi, Satoshi Watabe, Toshiya Ohta
Faculty of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. nakano@ees.kyushu-u.ac.jp
Rinshō Biseibutsu Jinsoku Shindan Kenkyūkai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology 2013It was developed that an anti-norovirus single-chain Fv (scFv) antibody for immunochromathographic test kit by using the phage displayed technique and biopanning. To obtain the scFv having wide reactivity for several norovirus genotypes, a mixture of recombinant norovirus capsid proteins, including 11 norovirus genotypes, was used for biopanning. Then, one anti-norovirus scFv antibody that recognized both of norovirus genogroups GI and GII was identified. An immunochromatographic test strip was constructed by using the scFv and demonstrated it to detect norovirus in stool samples. The immunochromatographic strip showed similar sensitivity to a commercially available one on which several monoclonal antibodies are included.
Citation
Michihiko Nakano, Keita Murofushi, Satoshi Watabe, Toshiya Ohta. Development of an anti-norovirus single-chain Fv for immunochromatographic test kit. Rinshō Biseibutsu Jinsoku Shindan Kenkyūkai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology. 2013;23(2):69-73
Mesh Tags
Substances
PMID: 23577605
View Full Text
