Levels of supramolecular chirality of polyglutamine aggregates revealed by vibrational circular dichroism.

Dmitry Kurouski, Karunakar Kar, Ronald Wetzel, Rina K Dukor, Igor K Lednev, Laurence A Nafie

FEBS letters 2013 Jun 05

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Polyglutamine (PolyQ) aggregates are a hallmark of several severe neurodegenerative diseases, expanded CAG-repeat diseases in which inheritance of an expanded polyQ sequence above a pathological threshold is associated with a high risk of disease. Application of vibrational circular dichroism (VCD) reveals that these PolyQ fibril aggregates exhibit a chiral supramolecular organization that is distinct from the supramolecular organization of previously observed amyloid fibrils. PolyQ fibrils grown from monomers with Q repeats 35 and above (Q≥35) exhibit approximately 10-fold enhancement of the same VCD spectrum compared to the already enhanced VCD of fibrils formed from Q repeats 30 and below (Q≤30). Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Citation

Dmitry Kurouski, Karunakar Kar, Ronald Wetzel, Rina K Dukor, Igor K Lednev, Laurence A Nafie. Levels of supramolecular chirality of polyglutamine aggregates revealed by vibrational circular dichroism. FEBS letters. 2013 Jun 05;587(11):1638-43