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Successful gene delivery into mammalian cells using bactofection requires entry of the bacterial vector via cell surface integrin receptors followed by release of plasmid DNA into the cellular environment. We show, for the first time, that addition of the DNA transfection reagent Lipofectamine improves entry of invasive Escherichia coli into HeLa cells and enhances up to 2.8-fold green fluorescent protein (GFP) expression from a reporter plasmid. The addition of Lipofectamine may be applicable to other bacterial vectors to increase their DNA delivery efficiency into mammalian cells. Copyright © 2013 Elsevier Inc. All rights reserved.


Kumaran Narayanan, Choon Weng Lee, Aurelian Radu, Edmund Ui Hang Sim. Escherichia coli bactofection using Lipofectamine. Analytical biochemistry. 2013 Aug 15;439(2):142-4

PMID: 23608053

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