Effects of the deletion of RsbV gene on virulence of Listeria monocytogenes].

Zaichao Zhang, Qingling Meng, Jun Qiao, Chuangfu Chen, Xuepeng Cai, Lihong Yang, Weisheng Wang, Kuojun Cai

Key Laboratory of the High Incidence of Local and National Disease of Ministry of' Education, Shihezi University, Shihezi 832003, China. zhangzaichao@sina.cn

Wei sheng wu xue bao = Acta microbiologica Sinica 2013 Jan 4

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To study the effect of RsbV gene deletion on the virulence of Listeria monocytogenes. The fragment with RsbV deletion was generated by gene overlap extension PCR (SOE-PCR) and the mutant with RsbV deletion was obtained by homologous recombination based on the wild strain LM-XS5. The differences in virulence between the two strains were determined by LD50, bacterial counts in liver and spleen, and qRT-PCR experiments. LD50 of the RsbV gene-deleted strain was 10(4) higher than that of the wild strain. The numbers of gene-deleted strain in the mouse's liver and spleen were significantly fewer than that of the wild strain (P < 0.05). Results of qRT-PCR show that four virulence factors' expression levels of the RsbV gene-deleted strain were significantly lower than that of the wild strain (P < 0.05). The RsbV gene-deleted strain induced a strong immune response in mice against the wild strain. RsbV regulates the expression of four virulence gene (inlA, LLO, PlcA and PrfA) of Listeria monocytogenes; The virulence of the RsbV gene-deleted strain is significantly reduced, but it still has good immunogenicity.

Citation

Zaichao Zhang, Qingling Meng, Jun Qiao, Chuangfu Chen, Xuepeng Cai, Lihong Yang, Weisheng Wang, Kuojun Cai. Effects of the deletion of RsbV gene on virulence of Listeria monocytogenes]. Wei sheng wu xue bao = Acta microbiologica Sinica. 2013 Jan 4;53(1):59-65