Detection of hepatitis A virus RNA with an improved loop-mediated isothermal amplification assay].

Feng Qiu, Min-Zhuo Guo, Jun-Ying Ding, Yao Yi, Zhi-Yuan Jia, Qiu-Dong Su, Sheng-Li Bi

National Institute of Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 102206, China.

Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology 2012 Dec

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To establish a novel improved loop-mediated isothermal amplification (LAMP) technique to detect hepatitis A virus (HAV). A novel improved LAMP assay based on the addition of an acceleration primer was developed for hepatitis A virus nucleotide acid detection. Precision and reproducibility analysis proved its high stability and reliability. Comparison between the improved and conventional LAMP assays revealed that the former was more sensitive with a detection limit of 5 TCID50/ml. The novel detection method displayed 100% consistency with the TaqMan real-time PCR assay when applied to clinical specimens collected from patients with confirmed acute HAV infection. This novel technique is widely applicable as a simple diagnostic tool in the clinical field as well as for the surveillance and investigation of the infectious disease in developing countries where HAV is endemic.

Citation

Feng Qiu, Min-Zhuo Guo, Jun-Ying Ding, Yao Yi, Zhi-Yuan Jia, Qiu-Dong Su, Sheng-Li Bi. Detection of hepatitis A virus RNA with an improved loop-mediated isothermal amplification assay]. Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology. 2012 Dec;26(6):483-5