The reduced pulmonary function is related to the decreased expression of Treg and Foxp3 in adjuvant-induced arthritis rats].

To observe changes of pulmonary function, regulatory T cells (Treg), forkhead transcription factor protein 3 (Foxp3) in adjuvant-induced arthritis (AA) rats, and explore the roles of Treg and Foxp3 expression in immunological mechanisms of AA. Thirty rats were randomly divided into normal control (NC) group and model control (MC) group, with 15 in each. The rats of MC group were induced by intracutaneous injection of 0.1 mL complete Freund's adjuvant in the right posterior paw. After 30 d, we observed the changes of the toe swelling degree, arthritis index. Pulmonary function was detected by animal spirometry, Treg in the peripheral blood by flow cytometry, and Foxp3 mRNA and protein by RT-PCR, immunohistochemistry, Western blotting, respectively. RESULTS Compared with the NC group, the toe swelling degree and arthritis index significantly increased, and the parameters of pulmonary function, CD4(+);CD25(+); Treg, CD4(+);CD25(+);FoxP3(+); Treg, Foxp3 mRNA and protein levels decreased in the MC group (P<0.01 or P<0.05). AA rats suffer from reduced pulmonary function and immune dysfunction, which may be related to the decreased expressions of Treg and Foxp3.

Citation

Lei Wan, Jian Liu, Chuanbing Huang, Yuan Wang, Lei Liu, Yuanyuan Cheng, Yunxia Feng. The reduced pulmonary function is related to the decreased expression of Treg and Foxp3 in adjuvant-induced arthritis rats]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 2013 Mar;29(3):251-5

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PMID: 23643080

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