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The objective of this study is to investigate whether F-box only protein 9 (FBXO9), an ubiquitination E3 ligase, has a functional role in adipocyte differentiation. Expression of FBXO9 was compared between obese mice and control lean mice using real-time PCR. Also, expression pattern of FBXO9 was monitored during 3T3-L1 adipocyte differentiation. FBXO9 was highly expressed in obese mice, and increased in the early stages of adipogenesis. To verify a functional role of FBXO9 in adipogenesis, FBXO9 was knocked down using transfection of siRNAs against FBXO9 into 3T3-L1 cells during the induction of adipogenesis. Knockdown of FBXO9 in early stage of adipogenesis almost completely inhibited adipogenesis, and CCAAT/enhancer binding protein β (C/EBPβ) levels were significantly reduced. However, the cells stably expressing C/EBPβ were fairly differentiated into adipocytes in the FBXO9 knockdown condition. These results suggest that FBXO9 is required for adipocyte differentiation, and C/EBPβ plays a role in the effect of FBXO9 on adipogenesis. Copyright © 2013 Elsevier Inc. All rights reserved.

Citation

Kyeong Won Lee, Soo Heon Kwak, Byung Yong Ahn, Hak Mo Lee, Hye Seung Jung, Young Min Cho, Young Joo Park, Sung Soo Chung, Kyong Soo Park. F-box only protein 9 is required for adipocyte differentiation. Biochemical and biophysical research communications. 2013 May 31;435(2):239-43

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PMID: 23643813

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