S Sekar, K Sasirekha, S Krishnakumar, T P Sastry
Bio-Products Laboratory, Central Leather Research Institute, Chennai, India.
Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine 2013 MarThe aim of this study was to evaluate the efficacy of Al2(SO4)3 cross-linked human amniotic membrane for ocular surface reconstruction using tissue culture techniques. The human amniotic membrane was cross-linked with Al2(SO4)3, and the cross-linked human amniotic membrane was characterized for its mechanical properties, percentage of swelling in water, sterility, infrared spectroscopy and scanning electron microscopy. The potential of cross-linked human amniotic membrane to support the attachment and proliferation of corneal limbal epithelial cells was assessed in vitro, using static culture system. About 125% increase in the tensile strength was observed in the cross-linked human amniotic membrane compared to human amniotic membrane. Infrared spectroscopy studies have confirmed the cross-linking of human amniotic membrane with Al2(SO4)3. The cross-linked human amniotic membrane was found to be sterile up to 1 year. In culture studies, confluent sheets of epithelial cells were seen at the end of 14th day resembling the morphological features of limbal epithelia. The cross-linked human amniotic membrane has exhibited improved mechanical properties, and the tissue culture studies have shown its feasibility to be used as a limbal transplant. It was concluded that the crosslinked human amniotic membrane with its improved mechanical properties could be used on par with human amniotic membrane.
S Sekar, K Sasirekha, S Krishnakumar, T P Sastry. A novel cross-linked human amniotic membrane for corneal implantations. Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine. 2013 Mar;227(3):221-8
PMID: 23662337
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