Yang Yue, Yi Sheng, Hai-Ning Zhang, Yong Yu, Lin Huo, Wei Feng, Tao Xu
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Biochemical and biophysical research communications 2013 Jun 7KIF1A, a member of kinesin-3 motors, plays a pivotal role in anterograde axonal transport of synaptic vesicles (SVs). We have shown that the CC1-FHA tandem of KIF1A forms a stable dimer that is crucial for both the dimerization and activation of the motor. However, it remains to be determined whether the CC1-FHA dimer is essential for KIF1A-mediated axonal transport in vivo. Here, we use Caenorhabditis elegans as the model organism to probe the in vivo function of the CC1-FHA dimer. Disruption of the CC1-FHA dimer severely impairs the KIF1A-mediated regulation of the locomotion and pumping behavior of C. elegans and exerts a significant impact on KIF1A-mediated axonal SV transport. Thus, together with previous structural and biochemical studies, the in vivo data presented in this study firmly establish the essential role of the CC1-FHA dimer for KIF1A-mediated neuronal transport. Copyright © 2013 Elsevier Inc. All rights reserved.
Yang Yue, Yi Sheng, Hai-Ning Zhang, Yong Yu, Lin Huo, Wei Feng, Tao Xu. The CC1-FHA dimer is essential for KIF1A-mediated axonal transport of synaptic vesicles in C. elegans. Biochemical and biophysical research communications. 2013 Jun 7;435(3):441-6
PMID: 23669038
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