Analysis of the role of vaccinia virus H7 in virion membrane biogenesis with an H7-deletion mutant.

Xiangzhi Meng, Xiang Wu, Bo Yan, Junpeng Deng, Yan Xiang

Journal of virology 2013 Jul

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Essential vaccinia virus genes are often studied with conditional-lethal inducible mutants. Here, we constructed a deletion mutant lacking the essential H7R gene (the ΔH7 mutant) with an H7-expressing cell line. Compared to an inducible H7 mutant, the ΔH7 mutant showed a defect at an earlier step of virion membrane biogenesis, before the development of short crescent-shaped precursors of the viral envelope. Our studies refine the role of H7 in virion membrane biogenesis and highlight the values of analyzing deletion mutants.

Citation

Xiangzhi Meng, Xiang Wu, Bo Yan, Junpeng Deng, Yan Xiang. Analysis of the role of vaccinia virus H7 in virion membrane biogenesis with an H7-deletion mutant. Journal of virology. 2013 Jul;87(14):8247-53