Lei Liu, Xiangan Han, Rui Liu, Hao Bai, Hongliang Dong, Chan Ding, Haiwen Liu, Shengqing Yu
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, China. liulei0606@gmail.com
Wei sheng wu xue bao = Acta microbiologica Sinica 2013 Mar 4Autoinducer 2 (AI-2), used to communicate among bacterial species, regulates numerous physiological functions of bacteria. In this study, we studied the effect of AI-2 on adherence and invasion of Riemerella antatipestifer (RA) strain CH3 to Vero cells and transcriptional levels of virulence-related and metabolism-related genes were investigated. To verify whether the adherence and invasion of CH3 was affected by AI-2, we added different concentrations of AI-2 to the cocultures of Vero cells and CH3 and then calculated adherence percentages and invasion percentages of tested groups. We further added AI-2 (184.0 micromol/L) to the tryptone soya broth culture of CH3 and then detected the effect of transcriptional levels of related genes of CH3 using real-time PCR. The adherence of CH3 to Vero cells was decreased most to 62% with 18.4 micromol/L AI-2 and the invasion of CH3 to Vero cells was increased most to 194% with 184.0 micromol/L AI-2. The result of real-time PCR shows that AI-2 increased transcriptional levels of some virulence-related genes and decreased transcriptional levels of some metabolism-related genes. These results suggest that AI-2 affected adherence and invasion of CH3 to Vero cells. Moreover, AI-2 could regulate some genes of CH3 to modulate particular physiological behaviors.
Lei Liu, Xiangan Han, Rui Liu, Hao Bai, Hongliang Dong, Chan Ding, Haiwen Liu, Shengqing Yu. Effect of autoinducer 2 on Riemerell antatipestifer adherence and invasion to Vero cells]. Wei sheng wu xue bao = Acta microbiologica Sinica. 2013 Mar 4;53(3):313-9
PMID: 23678579
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