Preparation and application of reversed phase Chromatorotor for the isolation of natural products by centrifugal preparative chromatography.

Ilias Muhammad, Volodymyr Samoylenko, Francis Machumi, Mohamed Ahmed Zakia, Rabab Mohammed, Mona H Hetta, Van Gillum

National Center for Natural Products Research, School of Pharmacy, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS 38677, USA. milias@olemiss.edu

Natural product communications 2013 Mar

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A method of preparation of rotors with a reversed phase (RP) solid silica gel sorbent layer has been developed for centrifugal preparative chromatography (CPC), also known as rotational planar chromatography (RPC). The rotors consist of binder free RP solid SiO2 layers of different thicknesses packed between two supported circular glass discs and can be used in any appropriate device for centrifugal chromatography, like Chromatotron and CycloGraph. Polar and /or semi-polar compounds with close R(f) values, as well as extracts and column fractions were separated and/or purified in a preparative and/or semi-preparative scale using the RP rotors, eluted with mixtures of aqueous-based solvents. We herein report three examples of its application, using RP Chromatorotors, for the isolation of the diastereoisomeric alkaloids banistenosides I and II from Banisteriopsis caapi, saponins III and IV from Fagonia cretica, and the sesquiterpenes artemisinin (V) and artemisinic acid (VI) from Artemisia annua.

Citation

Ilias Muhammad, Volodymyr Samoylenko, Francis Machumi, Mohamed Ahmed Zakia, Rabab Mohammed, Mona H Hetta, Van Gillum. Preparation and application of reversed phase Chromatorotor for the isolation of natural products by centrifugal preparative chromatography. Natural product communications. 2013 Mar;8(3):311-4