Determination of phospholipase C activity in vitro.

S M Teresa Hernández-Sotomayor, J Armando Muñoz-Sanchez

Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Merida, Yucatan, Mexico.

Methods in molecular biology (Clifton, N.J.) 2013

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Measurement of phospholipase C (PLC) activity in vitro is a valuable biochemistry technique easily applicable in samples from different organisms. It quantifies the enzymatic activity of a key protein involved in critical developmental functions in organisms such as plants, animals, and bacteria. A protocol is described which assays the formation of two main products of the PLC hydrolysis reaction on radioactively labeled phospholipid substrates.

Citation

S M Teresa Hernández-Sotomayor, J Armando Muñoz-Sanchez. Determination of phospholipase C activity in vitro. Methods in molecular biology (Clifton, N.J.). 2013;1009:187-92

PMID: 23681534

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