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Lipid affinity beads can be used to identify novel proteins with lipid binding capacity or to determine binding prerequisites of known lipid-binding proteins. Here we describe several applications for which this tool can be used and which considerations have to be taken into account. In addition to a precise protocol, several suggestions are made for experimental setups to facilitate identification of in vivo lipid binding targets.


Fionn McLoughlin, Christa Testerink. Lipid affinity beads: from identifying new lipid binding proteins to assessing their binding properties. Methods in molecular biology (Clifton, N.J.). 2013;1009:273-80

PMID: 23681542

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