Laura J Bray, Karina A George, Shuko Suzuki, Traian V Chirila, Damien G Harkin
School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia.
Methods in molecular biology (Clifton, N.J.) 2013Fibroin extracted from silkworm cocoon silk provides an intriguing and potentially important biomaterial for corneal reconstruction. In this chapter we outline our methods for producing a composite of two fibroin-based materials that support the cocultivation of human limbal epithelial (HLE) cells and human limbal stromal (HLS) cells. The resulting tissue substitute consists of a stratified epithelium overlying a three-dimensional arrangement of extracellular matrix components (principally "degummed" fibroin fibers) and mesenchymal stromal cells. This tissue substitute is currently being evaluated as a tool for reconstructing the corneal limbus and corneal epithelium.
Laura J Bray, Karina A George, Shuko Suzuki, Traian V Chirila, Damien G Harkin. Fabrication of a corneal-limbal tissue substitute using silk fibroin. Methods in molecular biology (Clifton, N.J.). 2013;1014:165-78
PMID: 23690012
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