Confocal microscopy as a tool for the study of the intranuclear topography of chromosomes.

H van Dekken, A van Rotterdam, R Jonker, H T van der Voort, G J Brakenhoff, J G Bauman

Radiobiological Institute TNO, Rijswijk, The Netherlands.

Journal of microscopy 1990 May

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A scanning confocal microscope was used to investigate the spatial positions of specific regions within blood cell nuclei. These centromeric regions were fluorescently labelled by in-situ hybridization to suspended nuclei with a centromere-1-specific DNA probe. The 3-D image data sets, obtained by optical sectioning of the cells, were used to determine the spatial position of the centromeric regions in the nuclei by means of specially developed software. The centromeres were found to be localized near the nuclear boundary. This spatial pattern was tested against a random distribution model by means of the Kolmogorov-Smirnov test. The difference between the two patterns was at a P less than 0.01 significance level.

Citation

H van Dekken, A van Rotterdam, R Jonker, H T van der Voort, G J Brakenhoff, J G Bauman. Confocal microscopy as a tool for the study of the intranuclear topography of chromosomes. Journal of microscopy. 1990 May;158(Pt 2):207-14