HSF2BP represses BNC1 transcriptional activity by sequestering BNC1 to the cytoplasm.

Yujian Wu, Shangying Liao, Xiuxia Wang, Si Wang, Min Wang, Chunsheng Han

State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

FEBS letters 2013 Jul 11

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Basonuclin (BNC1), a zinc finger transcriptional factor, is essential for mouse spermatogenesis. However, the regulatory mechanisms of BNC1 in spermatogenesis are poorly understood. In this study, we identified HSF2BP, a testis-specific binding protein of HSF2, as a binding partner of BNC1 by using yeast two-hybrid screening. HSF2BP could interact with and inhibit BNC1 transcriptional activity without affecting its expression level. Moreover, coexpression of HSF2BP with BNC1 resulted in a striking redistribution of BNC1 to the cytoplasm. These data suggest that HSF2BP may play a pivotal role in regulating BNC1 transcriptional activity and subcellular localization during spermatogenesis. Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Citation

Yujian Wu, Shangying Liao, Xiuxia Wang, Si Wang, Min Wang, Chunsheng Han. HSF2BP represses BNC1 transcriptional activity by sequestering BNC1 to the cytoplasm. FEBS letters. 2013 Jul 11;587(14):2099-104