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To develop the characteristic chromatographic profile of Sarcandra glabra by HPLC for its quality control. The HPLC analysis was performed on an Agilent Zorbax Eclipse XDB-C18 column (4. 6 mm x 250 mm, 5 microm) with column temperature at 40 degree C. The mobile phase was consisted of water containing 0. 5% formic acid and acetonitrile to methanol (1:9) in gradient mode, and the detection wavelength was set at 344 nm. A common mode of the HPLC characteristic chromatographic profile has been establised. There were 20 common peaks , seven of which were identified, and 46 samples from different habitats were classified into five groups based on principal component cluster analysis. The method was time-saving and can represent the chemical information and provide a scientific basis for quality control of S. glabra.


Pei-Ling Lin, Jian-Wei Zeng, Chun-Hua Ding, Yi-Chi Liang. HPLC characteristic chromatographic profile of Sarcandra glabra]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 2013 Mar;38(6):856-60

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PMID: 23717967

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