Retromer maintains basolateral distribution of the type II TGF-β receptor via the recycling endosome.

Xueqian Yin, Stephen J Murphy, Mark C Wilkes, Yan Ji, Edward B Leof

Molecular biology of the cell 2013 Jul

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Transforming growth factor β (TGF-β) is critical for the development and maintenance of epithelial structures. Because receptor localization and trafficking affect the cellular and organismal response to TGF-β, the present study was designed to address how such homeostatic control is regulated. To that end, we identify a new role for the mammalian retromer complex in maintaining basolateral plasma membrane expression of the type II TGF-β receptor (TβRII). Retromer and TβRII associate in the presence or absence of TGF-β ligand. After retromer knockdown, although TβRII internalization and trafficking to a Rab5-positive compartment occur as in wild-type cells, receptor recycling is inhibited. This results in TβRII mislocalization from the basolateral to both the basolateral and apical plasma membranes independent of Golgi transit and the Rab11-positive apical recycling endosome. The data support a model in which, after initial basolateral TβRII delivery, steady-state polarized TβRII expression is maintained by retromer/TβRII binding and delivery to the common recycling endosome.

Citation

Xueqian Yin, Stephen J Murphy, Mark C Wilkes, Yan Ji, Edward B Leof. Retromer maintains basolateral distribution of the type II TGF-β receptor via the recycling endosome. Molecular biology of the cell. 2013 Jul;24(14):2285-98