Determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets by HPLC isocratic elution].

To establish an HPLC method for determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets. HPLC isocratic elution was adopted, with the C18 column (4.6 mm x 250 mm, 5 microm) as the chromatographic column, methanol-water-phosphoric acid (45: 55: 0.2) as the mobile phase. The flow rate was set at 1.0 mL x min(-1), and column temperature was set at 30 degrees C. The detection wavelength was 328 nm, and the sample size was 20 microL. At concentrations ranging between 2.200-44.00, 24.20-242.0 mg x L(-1), peak area of chlorogenic acid and baicalin showed good linear relationship, the average recoveries were 99.63% (RSD 1.8%, n = 9) and 99.72% (RSD 1.9%, n = 9), respectively. The method is simple that it can accurately measure the content of chlorogenic acid and baicalin in Yinhuang film-coated tablets.

Citation

Mei Xu, Qi Yuan, Hui Zhao, Fan Li, Xiao-Lei Hu, Ying-Ying Fu, Guo-Hua Shi, Xiao-Ming Wang, Na Zhang. Determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets by HPLC isocratic elution]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 2013 Mar;38(5):691-3

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PMID: 23724677

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