Qin Chu, Shi-Hui Jiao, Ya-Chun Wang, Lin Liu, Ai-Rong Liu, Hong-Jun Wu, Zhen-Quan Xie, Shi-Yu Hou, Fan-Jun Geng, Cong-Yong Wang, Xi-Xia Huang, Shi-Xin Tan, Rui Tan, Yi Zhang, Ying Yu, Yuan Zhang
Beijing Academy of Agriculture and Forestry Sciences, Beijing, China. chuqinsd@163.com
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji 2013 MayArachnomelia syndrome (AS) is a recessive inherited disease in cattle. Although the arachnomelia phenotypes are virtually identical in Brown Swiss and Simmental cattle, the causative mutation are different, which are a 1 bp insertion c.363-364insG in the sulfite oxidase (SUOX) gene and a 2 bp deletion c.1224_1225delCA in the molybdenum cofactor syn-thesis step 1 (MOCS1) gene, respectively. In the current study, combining fluorescence PCR with capillary electrophoresis technology, an automatic fluorescence method was established, which could detect the two causative loci rapidly and cor-rectly with a single reaction. Samples from 51 Simmental bulls, 80 cows mated artificially using semen of Simmental bulls and their resulted 106 progeny, together with 55 Xinjiang Brown were collected and used for validation of the newly de-signed methods. Our results have laid a foundation for screening AS disease causing mutations in Chinese cattle.
Qin Chu, Shi-Hui Jiao, Ya-Chun Wang, Lin Liu, Ai-Rong Liu, Hong-Jun Wu, Zhen-Quan Xie, Shi-Yu Hou, Fan-Jun Geng, Cong-Yong Wang, Xi-Xia Huang, Shi-Xin Tan, Rui Tan, Yi Zhang, Ying Yu, Yuan Zhang. Establishment of the detection method for two causative genes of cattle arachnomelia syndrome]. Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji. 2013 May;35(5):623-7
PMID: 23732669
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