Correlation Engine 2.0
Clear Search sequence regions

  • APC (4)
  • cdc2 protein (2)
  • Cdc20 (1)
  • cdc25 (2)
  • Cdc55 (3)
  • Cdk1 (5)
  • cell cycle (2)
  • genes cdc (1)
  • kinases (2)
  • MIH1 (1)
  • morphogenesis (2)
  • ras GRF1 (2)
  • subunit protein (1)
  • SWE1 (5)
  • Wee1 (3)
  • yeast (2)
  • Sizes of these terms reflect their relevance to your search.

    Cdk1 drives both mitotic entry and the metaphase-to-anaphase transition. Past work has shown that Wee1 inhibition of Cdk1 blocks mitotic entry. Here we show that the budding yeast Wee1 kinase, Swe1, also restrains the metaphase-to-anaphase transition by preventing Cdk1 phosphorylation and activation of the mitotic form of the anaphase-promoting complex/cyclosome (APC(Cdc20)). Deletion of SWE1 or its opposing phosphatase MIH1 (the budding yeast cdc25(+)) altered the timing of anaphase onset, and activation of the Swe1-dependent morphogenesis checkpoint or overexpression of Swe1 blocked cells in metaphase with reduced APC activity in vivo and in vitro. The morphogenesis checkpoint also depended on Cdc55, a regulatory subunit of protein phosphatase 2A (PP2A). cdc55Δ checkpoint defects were rescued by mutating 12 Cdk1 phosphorylation sites on the APC, demonstrating that the APC is a target of this checkpoint. These data suggest a model in which stepwise activation of Cdk1 and inhibition of PP2A(Cdc55) triggers anaphase onset.


    Noel Lianga, Elizabeth C Williams, Erin K Kennedy, Carole Doré, Sophie Pilon, Stéphanie L Girard, Jean-Sebastien Deneault, Adam D Rudner. A Wee1 checkpoint inhibits anaphase onset. The Journal of cell biology. 2013 Jun 10;201(6):843-62

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 23751495

    View Full Text