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The coumarins: scopoletin, esculetin and fraxetin were isolated from the flowers of horse chestnut (Aesculus hippocastanum L., Hippocastanaceae) and identified by spectrophotometric methods (UV, 1H, 13C NMR, ESI-MS). Their content, determined using the Ultra Performance Liquid Chromatography (UPLC), was 0.41, 0.13 and 0.05%, respectively.


Marlena Dudek-Makuch, Irena Matławska. Coumarins in horse chestnut flowers: isolation and quantification by UPLC method. Acta poloniae pharmaceutica. 2013 May-Jun;70(3):517-22

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PMID: 23757942

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