Aerobic methylobacteria as the basis for a biosensor for dichloromethane detection].

Iu V Plekhanova, Iu E Firsova, N V Doronina, Iu A Trotsenko, A N Reshetilov

Prikladnaia biokhimiia i mikrobiologiia 2013 Mar-Apr

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Cells of dichloromethane (DChM) bacteria-destructors were immobilized by sorption on different types of membranes, which were fixed on the measuring surface of a pH-sensitive field transistor. The presence of DChM in the medium (0.6-8.8 mM) led to a change in the transistor's output signal, which was determined by the appearance of H+ ions in the medium due to DChM utilization by methylobateria. Among four strains of methylobacteria--Methylobacterium dichloromethanicum DM4, Methylobacterium extorquens DM 17, Methylopila helvetica DM6, and Ancylobacter dichloromethanicus DM 16--the highest and most stable activity toward DChM degradation was observed in the strain M. dichloromethanicum DM4. Among 11 types of membranes for cell immobilization, Millipore nitrocellulose membranes and chromatographic fiber paper GF/A, which allow one to obtain stable biosensor signals for 2 weeks without a bioreceptor change, were chosen as optimal carriers.

Citation

Iu V Plekhanova, Iu E Firsova, N V Doronina, Iu A Trotsenko, A N Reshetilov. Aerobic methylobacteria as the basis for a biosensor for dichloromethane detection]. Prikladnaia biokhimiia i mikrobiologiia. 2013 Mar-Apr;49(2):203-8