Screening of differentially expressed proteins in serum from subjects with Keshan disease by two-dimensional electrophoresis and mass and mass spectrometry].

Shulan He, Wuhong Tan, Jing Wang, Pan Wang, Youzhang Xiang

Wei sheng yan jiu = Journal of hygiene research 2013 May

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To screen differentially expressed proteins in serum in patients with Keshan disease (KD), peripheral blood protein expression spectrum between subjects with Keshan disease and health controls were compared. Differentially expressed protein spots were screened by two-dimensional gel electrophoresis (2-DE) between Keshan disease and health control subjects, and constitutive protein were identified by matrix assisted laser adsorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). 9 differentially expressed protein spots were showed in 2-DE images and 8 differentially expressed proteins were identified by MALDI-TOF-MS. In them, 3 up-regulated proteins, mainly relatedd to lipid metabolism, apoptosis resistance, immunological regulation and 3 down-regulated proteins, involved to cellular iron ion homeostasis; 2 up-regulated proteins in serum in patients with KD versus controls from KD areas were detected, mainly associated with protease inhibition. Haptoglobin, serum albumin, alpha-l-antitrypsin, transferring and alpha2-heremans schmid glycoprotein may be considered as candidate biological indicators used for diagnosis or prognosis of KD.

Citation

Shulan He, Wuhong Tan, Jing Wang, Pan Wang, Youzhang Xiang. Screening of differentially expressed proteins in serum from subjects with Keshan disease by two-dimensional electrophoresis and mass and mass spectrometry]. Wei sheng yan jiu = Journal of hygiene research. 2013 May;42(3):424-8