The verification possibilities of immunochips under low content of antibodies to Core-antigen of virus of hepatitis C].

A A Potapova, T A Chekanova, M L Markelov, E A Pudova, N P Kirdiashkina, G A Shipulina, V M Dronova, N N Potekaeva

Klinicheskaia laboratornaia diagnostika 2013 Jan

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The detection of antibodies to Core-antigen of virus of hepatitis C in test-systems for solid-phase immune-enzyme analysis with low optical density can be a result not only of true availability of antibodies but an effect of nonspecific reaction of blood serum. The diagnostic possibilities of immunochips to be used in immune-enzyme analysis for verification of availability of markers of viral hepatitis C were investigated in conditions of low positive reaction of blood serum to core-antigen. It is established that immunochips and immunoblots have similar specificity concerning detection of antibodies to Core-antigen. At that, in immunochips antibodies to nonstructural antigens of virus of hepatitis C were additionally detected in more than 90% of samples.

Citation

A A Potapova, T A Chekanova, M L Markelov, E A Pudova, N P Kirdiashkina, G A Shipulina, V M Dronova, N N Potekaeva. The verification possibilities of immunochips under low content of antibodies to Core-antigen of virus of hepatitis C]. Klinicheskaia laboratornaia diagnostika. 2013 Jan(1):45-8