A mass-differentiated library strategy for identification of sugar nucleotidyltransferase activities from cell lysates.

Kwang-Seuk Ko, Rahman M Mizanur, Joy M Jackson, Lin Liu, Nicola L B Pohl

Department of Chemistry, Department of Chemical and Biological Engineering, and Plant Sciences Institute, Iowa State University, Ames, IA 50011, USA.

Analytical biochemistry 2013 Oct 1

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Sugar nucleotidyltransferases, or nucleotide sugar pyrophosphorylases, are ubiquitous enzymes whose activities have been correlated to disease states and pathogen virulence. Here we report a rapid "one-pot" method to identify a range of sugar nucleotidyltransferase activities of purified proteins or in cell lysates using a mass-differentiated carbohydrate library designed for mass spectrometry-based analysis. Copyright © 2013 Elsevier Inc. All rights reserved.

Citation

Kwang-Seuk Ko, Rahman M Mizanur, Joy M Jackson, Lin Liu, Nicola L B Pohl. A mass-differentiated library strategy for identification of sugar nucleotidyltransferase activities from cell lysates. Analytical biochemistry. 2013 Oct 1;441(1):8-12

PMID: 23811154

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