Long Acting Propranolol and HSP-70 Rich Tumor Lysate Reduce Tumor Growth and Enhance Immune Response against Fibrosarcoma in Balb/c Mice.

Background: Noradrenaline (NA), the principal neurotransmitter released from sympathetic nerve terminals, influences T-cell maturation, not only directly in developing T cells, but also indirectly, by acting on the thymic nonlymphoid cells. In vitro and in vivo studies have demonstrated the anti-proliferative, anti-migratory, anti-angiogenic and cytotoxic properties of propranolol, β-AR blocker, against various cancers. Objectives: To evaluate the effect of propranolol on efficacy of HSP-70 rich lysate vaccine in immunotherapy of fibrosarcoma. Methods: Mouse fibrosarcoma WEHI-164 cells were used to immunize tumor-bearing mice with or without propranolol and HSP-70. Splenocytes proliferation, cytotoxicity activity of the splenocytes, naturally occurring CD4+ CD25high T-reg cells and IFN-γ and IL-4 secretion as well as tumor size, were assessed to describe the anti-tumor immune response. Results: A significant increase in the level of IFN-γ in the mice vaccinated with WEHI-164 cells enriched with HSP-70 and co-treated with propranolol was observed compared to controls. However, HSP enrichment or propranolol treatment alone did not enhance the immune response as measured by the level of IFN-γ. Likewise, a decrease in tumor growth in the test group (p<0.01) and a significant increase in CTL activity (p<0.05) was observed. Conclusion: HSP enriched vaccine shows anti-tumor activity, probably due to the modulation of immune responses.

Citation

Ahmad Khalili, Zuhair Muhammad Hassan, Shahram Shahabi, Ali Akbar Pourfathollah, Seyed Nasser Ostad, Shokoofe Noori, Mehdi Mahdavi, Habib Haybar, Ladan Langroudi. Long Acting Propranolol and HSP-70 Rich Tumor Lysate Reduce Tumor Growth and Enhance Immune Response against Fibrosarcoma in Balb/c Mice. Iranian journal of immunology : IJI. 2013 Jun;10(2):70-82

PMID: 23811546

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