Screening and evaluation of anti-respiratory syncytial virus compounds in cultured cells.

Respiratory syncytial virus (RSV) is a highly contagious pathogen that infects mainly ciliated cells of respiratory epithelium and type 1 pneumocytes in the alveoli frequently causing serious respiratory disease in infants, elderly, and immunocompromised patients. At present, prevention/treatment of RSV infection is limited to the use of specific anti-RSV antibody or an aerosol formulation of ribavirin, a drug of suboptimal efficacy and low safety profile. There is an urgent need for development of novel anti-RSV drugs and virucides. Here we describe the cell culture-based methods used in our laboratory in identification of novel inhibitors of RSV including the P13 fusion inhibitor, and the PG545 virucide. Protocols for antiviral screening, evaluation of anti-RSV potency, and elucidation of mode of antiviral activity of test compounds are described.

Citation

Anna Lundin, Tomas Bergström, Edward Trybala. Screening and evaluation of anti-respiratory syncytial virus compounds in cultured cells. Methods in molecular biology (Clifton, N.J.). 2013;1030:345-63

PMID: 23821281

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