Microbiological evaluation of ultrasonic nebulization for disinfecting dental impressions.

Marcio Jose Mendonca, Renata Santos Rafael, Veridiana Camilotti, Rafael Andrade Menolli, Eliseu Augusto Sicoli, Nancielli Teixeira, Mario Alexandre Coelho Sinhoreti

General dentistry 2013 Jul

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Disinfecting dental impressions is necessary to decrease the risk of cross-contamination in dental offices. Ultrasonic nebulization has been mentioned as a microbicidal technique that can be used to disinfect contaminated dental impressions. This study compared the microbicidal effect of 2% glutaraldehyde and 0.2% peracetic acid for the disinfection of dental impressions made with vinyl polysiloxane, using 2 disinfection methods: immersion and ultrasonic nebulization. Bactericial efficacy was examined using Staphylococcus aureus and Bacillus atrophaeus as indicators. Thirty impressions were obtained and distributed randomly in 5 groups (n = 6). Group 1 was immersed in 2% glutaraldehyde immersion for 10 minutes, Group 2 was immersed in 0.2% peracetic acid for 10 minutes, Group 3 underwent ultrasonic nebulization for 10 minutes in 2% glutaraldehyde solution, Group 4 underwent ultrasonic nebulization for 10 minutes in 0.2% peracetic acid solution, and Group 5 was a control group that received no disinfectant. Both solutions experienced a 100% reduction in microorganisms following ultrasonic nebulization, as did peracetic acid following immersion; however, immersion in glutaraldehyde demonstrated lower values of reduction in B atrophaeus group, with a statistically significant difference compared with the other experimental groups.

Citation

Marcio Jose Mendonca, Renata Santos Rafael, Veridiana Camilotti, Rafael Andrade Menolli, Eliseu Augusto Sicoli, Nancielli Teixeira, Mario Alexandre Coelho Sinhoreti. Microbiological evaluation of ultrasonic nebulization for disinfecting dental impressions. General dentistry. 2013 Jul;61(4):10-3