Brahma‑related gene 1-associated expression of 9-27 and IFI-27 is involved in acquired cisplatin resistance of gastric cancer cells.

In order to investigate the mechanism of cisplatin resistance, a cisplatin‑resistant human gastric cancer cell line was established. Subsequent to the exposure of the YCC-3 gastric cancer cell line to equal concentrations of cis‑diammine‑dichloroplatinum (II) (cisplatin, CDDP) for 6 months, a cisplatin-resistant cell line was established (YCC‑3/R). To determine the molecular mechanism of cisplatin resistance in YCC‑3/R cells, differentially expressed genes (DEGs) were investigated between YCC‑3 and YCC-3/R by annealing control primer-based reverse transcriptase-polymerase chain reaction (ACP RT‑PCR) technology. Eleven DEGs were successfully identified and sequenced. Among them, interferon‑induced transmembrane protein 1 (9‑27) and interferon α‑inducible protein 27 (IFI‑27) were markedly increased in YCC‑3/R cells. In addition, western blot analysis demonstrated that the Brahma‑related gene 1 (BRG1), which was observed to selectively activate 9‑27 and IFI‑27 genes, was overexpressed in YCC‑3/R cells. The results suggested that the BRG1‑associated expression of 9‑27 and IFI‑27 is involved in cisplatin resistance in gastric cancer cells.

Citation

Hye Ran Lee, Hyun Kyung No, Chun Jeih Ryu, Hyun-Ju Park. Brahma‑related gene 1-associated expression of 9-27 and IFI-27 is involved in acquired cisplatin resistance of gastric cancer cells. Molecular medicine reports. 2013 Sep;8(3):747-50

PMID: 23836109

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