Effects of human umbilical cord mesenchymal stem cells on the proliferation of hematopoietic malignancies].

To study the effects of human umbilical cord mesenchymal stem cells (UC-MSCs) on proliferation of eight tumor cell lines from leukemia, lymphoma and multiple myeloma in vitro. Tumor cells were labeled with carboxyfluorescein diacetate succinimidyl ester (CFDA-SE). UC-MSCs were co-cultured with tumor cells at different ratios as experimental groups, meanwhile, tumor cells were cultured without UC-MSCs as control groups. After three days, mean fluorescence intensity and cell division generations of the tumor cells were measured by flow cytometry. UC-MSCs inhibited the proliferation of HL60, THP1, K562 and RPMI8226 cell lines, but promoted the proliferation of Raji and NCIH929 cell lines. UC-MSCs promoted the proliferation of Jurkat cells only at 1:1 ratio; as for U937 cells, UC-MSCs inhibited the proliferation at 2:1 ratio (UC-MSCs: U937), promoted proliferation at 1:4 and 1:16, whereas had no obvious effect at 1:1. UC-MSCs have different effects on the proliferation of different hematopoietic tumor cell lines. They have no promoting effects on four leukemic cell lines, but have bidirectional (promotion/inhibition) effects on lymphoma and multiple myeloma cell lines. The U937 cell line may serve as a good model for the mechanism study of this contradictory phenomenon.

Citation

Yilin Pang, Yongyong Tang, Qian Li, Jing Xie, Xuefeng Zang, Tingting Liu, Bin Zhang, Hu Chen. Effects of human umbilical cord mesenchymal stem cells on the proliferation of hematopoietic malignancies]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 2013 Jul;29(7):714-8

Mesh Tags

PMID: 23837982

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