Laetitia Hennekinne, Sabrina Colasse, Antoine Triller, Marianne Renner
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Jul 10Thrombospondin-1 (TSP-1) is a large extracellular matrix protein secreted by astrocytes during development and inflammation. In the developing CNS, TSP-1 is involved in neuronal migration and adhesion, neurite outgrowth, and synaptogenesis. We investigated the effects of TSP-1 on neurons with mature synapses using immunocytochemistry, single-particle tracking, surface biotinylation, and calcium imaging. We show that in cultured rat spinal cord neurons TSP-1 decreased neuronal excitability by reducing the accumulation of excitatory AMPA receptors (AMPARs) and increasing that of inhibitory glycine receptors (GlyRs) in synapses. The effects of TSP-1 on GlyRs were dependent on the activation of excitatory receptors. These changes were abolished by blocking β1-integrins and mimicked by blocking β3-integrins. In the presence of TSP-1, AMPARs were less stabilized at synapses, increasing their lateral diffusion and endocytosis. Interestingly, TSP-1 counteracted the increased neuronal excitability and neuronal death induced by TNFα. These results suggest a role of TSP-1 in controlling the balance between excitation and inhibition which could help the recovery of normal synaptic activity after injury responses.
Laetitia Hennekinne, Sabrina Colasse, Antoine Triller, Marianne Renner. Differential control of thrombospondin over synaptic glycine and AMPA receptors in spinal cord neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2013 Jul 10;33(28):11432-9
PMID: 23843515
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