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The purpose of this study was to investigate the application of wide-field laser ophthalmoscopy (Optos) for the evaluation of established models of angiogenesis and the healthy retina in mice. To investigate whether angiogenesis and leakage in the retina and choroid can be evaluated with Optos, we used two models of angiogenesis: oxygen-induced retinopathy (OIR) and laser-induced choroidal neovascularization (CNV). Fundus imaging and fluorescein angiography (FAG) were performed with the Optos system without a contact lens. Furthermore, to evaluate in vivo leukocyte infiltration in these models, we injected acridine orange (AO) and performed imaging using Optos. In vivo fundus imaging with Optos did not require any additional optical device. Additionally, Optos enabled us to repeatedly obtain high-resolution color images and FAG images in the OIR model as well as in the CNV model in mice. Through a combination of Optos imaging and AO fluorography, the number and location of the infiltrating leukocytes could be identified in these models. Optos is a wide-viewing imaging tool for the noninvasive in vivo evaluation of common angiogenesis models, oxygen-induced retinopathy and laser-induced choroidal neovascularization, as well as the healthy retina in mice.


Shintaro Nakao, Ryoichi Arita, Takahito Nakama, Hiroshi Yoshikawa, Shigeo Yoshida, Hiroshi Enaida, Ali Hafezi-Moghadam, Takaaki Matsui, Tatsuro Ishibashi. Wide-field laser ophthalmoscopy for mice: a novel evaluation system for retinal/choroidal angiogenesis in mice. Investigative ophthalmology & visual science. 2013 Aug;54(8):5288-93

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PMID: 23860759

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