Youngkyun Lee, Hyo Jeong Kim, Cheol Kyu Park, Yong-Gun Kim, Heon-Jin Lee, Jae-Young Kim, Hong-Hee Kim
Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 700-412, Republic of Korea. Electronic address: ylee@knu.ac.kr.
Bone 2013 OctOsteoclasts are specialized cells for bone-resorption originated from precursors of macrophage/monocyte lineage. The receptor activator of NFκB ligand (RANKL) initiates osteoclast differentiation, in which nuclear factor of activated T cell cytoplasmic 1 (NFATc1) plays a key role as a master transcription factor. In the present report, we show that microRNA-124 (miR-124) regulates osteoclastogenesis of mouse bone marrow macrophages (BMMs) by suppressing NFATc1 expression. On the other hand, synthetic inhibitor that binds specifically to miR-124 enhanced osteoclast differentiation and NFATc1 expression. The overexpression of a constitutively active form of NFATc1 prevented the inhibitory effect of miR-124 on osteoclastogenesis. Finally, miR-124 also affected the proliferation and motility of osteoclast precursors, the latter coinciding with the reduced expression of RhoA and Rac1. These findings not only reveal unprecedented role of miR-124 in osteoclastogenesis but also suggest a novel mode of regulation of NFATc1 in osteoclasts. © 2013.
Youngkyun Lee, Hyo Jeong Kim, Cheol Kyu Park, Yong-Gun Kim, Heon-Jin Lee, Jae-Young Kim, Hong-Hee Kim. MicroRNA-124 regulates osteoclast differentiation. Bone. 2013 Oct;56(2):383-9
PMID: 23867221
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