Sasa R Savic, Zivomir B Petronijevic
Faculty of Technology, University of Nis, Bulevar Oslobodjenja 124, 16000 Leskovac, Serbia.
Indian journal of biochemistry & biophysics 2013 JunThe kinetic mechanism of enzymatic modification of flavonol quercetin with L-cysteine by horseradish peroxidase (HRP) was studied. Reaction of modification of quercetin was followed by recording spectral changes over time at 380 nm. All reactions were performed in 100 mM phosphate buffer pH, 6.0 at 20 degrees C. Kinetic parameters were determined from graphics of linear Michaelis-Menten equation. The values obtained at specified intervals were: V(max) = 0.17 divided by 0.91 deltaA380/min, K(m) = 0.023 divided by 0.5 mM, k(cat) = 0.21 divided by 1.14 deltaA380/min x nM(-1) and V(max)/K(m) = 0.83 divided by 26.55 deltaA380/min x mM(-1). It was found that all investigated reactions of the modification of quercetin with L-cysteine by HRP followed an ordered mechanism. We propose that HRP initially reacts with H2O2 than with quercetin and finally with L-cysteine, leading to the introduction of L-cysteine in the structure of quercetin.
Sasa R Savic, Zivomir B Petronijevic. Kinetics of enzymatic modification of quercetin with cysteine by horseradish peroxidase. Indian journal of biochemistry & biophysics. 2013 Jun;50(3):221-6
PMID: 23898486
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