The hepatitis B x antigen anti-apoptotic effector URG7 is localized to the endoplasmic reticulum membrane.
A Ostuni, P Lara, M F Armentano, R Miglionico, A M Salvia, M Mönnich, M Carmosino, F M Lasorsa, M Monné, I Nilsson, F Bisaccia
Department of Sciences, University of Basilicata, 85100 Potenza, Italy.
FEBS letters 2013 Sep 17Hepatitis B x antigen up-regulates the liver expression of URG7 that contributes to sustain chronic virus infection and to increase the risk for hepatocellular carcinoma by its anti-apoptotic activity. We have investigated the subcellular localization of URG7 expressed in HepG2 cells and determined its membrane topology by glycosylation mapping in vitro. The results demonstrate that URG7 is N-glycosylated and located to the endoplasmic reticulum membrane with an Nlumen-Ccytosol orientation. The results imply that the anti-apoptotic effect of URG7 could arise from the C-terminal cytosolic tail binding a pro-apoptotic signaling factor and retaining it to the endoplasmic reticulum membrane. Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Citation
A Ostuni, P Lara, M F Armentano, R Miglionico, A M Salvia, M Mönnich, M Carmosino, F M Lasorsa, M Monné, I Nilsson, F Bisaccia. The hepatitis B x antigen anti-apoptotic effector URG7 is localized to the endoplasmic reticulum membrane. FEBS letters. 2013 Sep 17;587(18):3058-62
PMID: 23912081
View Full Text
