PCR detection of the 14.5 antibacterial NlpC/P60-like Dermatophagoides pteronyssinus protein in Dermatophagoides farinae (Acari: Pyroglyphidae).

Tomas Erban, Cosimo Antonio Di Presa, Jan Kopecky, Palmiro Poltronieri, Jan Hubert

Department of Pest Control of Stored Products and Food Safety, Laboratory of Proteomics, Crop Research Institute, Drnovska507/73, Praha 6 Ruzyne, CZ-161 06, Czechia. arachnid@centrum.cz

Journal of medical entomology 2013 Jul

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House dust mites produce antibacterial proteins suppressing bacterial growth. The 14.5-kDa bacteriolytic protein (UniProtKB Q8MWR6) has been known in Dermatophagoides pteronyssinus Trouessart. We have applied polymerase chain reaction and reverse transcription-PCR to detect a homologous gene sequence coding for a Q8MWR6-related protein in Dermatophagoides farinae (Hughes) using genomic DNA and total RNA, respectively. The resulting PCR product of expected size, 243 bp, was obtained from both Dermatophagoides spp., while no amplification was achieved from stored product mite samples. Sequence of the gene fragment from D. farinae showed 83% similarity to the previously described one in D. pteronyssinus. Successful amplification of the expected product from cDNA generated with oligo-dT primer implies that the NlpC/P60-like protein in Dermatophagoides mites is of eukaryotic or mite origin.

Citation

Tomas Erban, Cosimo Antonio Di Presa, Jan Kopecky, Palmiro Poltronieri, Jan Hubert. PCR detection of the 14.5 antibacterial NlpC/P60-like Dermatophagoides pteronyssinus protein in Dermatophagoides farinae (Acari: Pyroglyphidae). Journal of medical entomology. 2013 Jul;50(4):931-3