Sensitive label-free monitoring of protein kinase activity and inhibition using ferric ions coordinated to phosphorylated sites as electrocatalysts.
Min Wang, Gui-Xia Wang, Fang-Nan Xiao, Yun Zhao, Kang Wang, Xing-Hua Xia
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. xhxia@nju.edu.cn.
Chemical communications (Cambridge, England) 2013 Sep 5Ferric ions show high affinity to the kinase-generated phosphorylated sites of peptides. Thus, the high electrocatalytic activity of the coordinated ferric ions toward the reduction of hydrogen peroxide can be used for sensitive label-free monitoring of protein kinase activity and inhibition.
Citation
Min Wang, Gui-Xia Wang, Fang-Nan Xiao, Yun Zhao, Kang Wang, Xing-Hua Xia. Sensitive label-free monitoring of protein kinase activity and inhibition using ferric ions coordinated to phosphorylated sites as electrocatalysts. Chemical communications (Cambridge, England). 2013 Sep 5;49(78):8788-90
PMID: 23958636
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