Juan Daniel Diaz-Valencia, Megan Bailey, Jennifer L Ross
Department of Physics, University of Massachusetts Amherst, Amherst, Massachusetts, USA.
Methods in cell biology 2013Microtubule-severing enzymes are a novel class of microtubule regulators. They are enzymes from the ATPases associated with various cellular activities family (AAA+) that utilize ATP to cut microtubules into smaller filaments. Discovered over 20 years ago, there are still many open questions about severing enzymes. Both cellular and biochemical studies need to be pursued to fully understand how these enzymes function mechanistically in the cell. Here, we present methods to express, purify, and test the biophysical nature of these proteins in vitro to begin to address the biochemical and biophysical mechanisms of this important and novel group of microtubule destabilizers. Copyright © 2013 Elsevier Inc. All rights reserved.
Juan Daniel Diaz-Valencia, Megan Bailey, Jennifer L Ross. Purification and biophysical analysis of microtubule-severing enzymes in vitro. Methods in cell biology. 2013;115:191-213
PMID: 23973074
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