Induction of somatic embryogenesis in endangered butterfly ginger Hedychium coronarium J. Koenig.

Plant Tissue Culture Laboratory, Department of P.G. Studies and Research in Biological Science, R. D University, Jabalpur 482 001, India.

Indian journal of experimental biology 2012 Dec

filter terms:

An efficient protocol has been developed for regeneration of complete plants through somatic embryogenesis in H. coronarium. Creamish white, pale yellow and brown calli were obtained on MS medium supplemented with different concentrations of auxins [2, 4-Dichlorophenoxy acetic acid (2, 4-D), Indole-3 acetic acid (IAA) and 1-Naphthylacetic acid (NAA)] after 4 weeks. Creamy white calli developed on 0.5 mg L(-1) 2, 4-D turned embryogenic when subcultured on basal medium and produced small globular somatic embryos after 6 weeks. Further growth of somatic embryos required their transfer to medium containing 6-benzylaminopurine (BAP) or kinetin (KN). BAP was more effective than KN in promoting shoot proliferation. Maximum shoot length was obtained with 0.5 mg L(-1) BAP whereas maximum shoot number was obtained with 1.0 mg L(-1) BAP. The plantlets thus formed were successfully hardened, and transferred to sand-soil and farm yard manure (1:1:1) with 95% survival.

Citation

Manju Verma, Y K Bansal. Induction of somatic embryogenesis in endangered butterfly ginger Hedychium coronarium J. Koenig. Indian journal of experimental biology. 2012 Dec;50(12):904-9