Quishui Li, Feng Shen, Xiao Zhang, Yufeng Hu, Qingxi Zhang, Lin Xu, Xueqin Ren
Department of Plant Nutrition, College of Resources and Environmental Sciences, China Agricultural University, Beijing, PR China.
Analytica chimica acta 2013 Sep 17The tyrosine phosphorylation of proteins plays a vital role in signal transduction pathways. The highly selective enrichment of tyrosine phosphopeptides remains a significant challenge in this area of research because of the low levels of tyrosine phosphorylation in cells. Herein, we report the development of a novel molecularly imprinted polymer (MIP)-based method for the recognition of tyrosine phosphopeptides in aqueous media using an epitope approach. Phenylphosphonic acid, which has been regarded the "epitope" of phosphotyrosine, was used as a template, and commercially available zinc acrylate was used as a functional monomer to prepare the MIP. The one-pot synthetic process was simple, efficient, and the resulting MIPs were low-cost, robust, and recyclable. The MIP demonstrated significant higher levels of adsorption capacity and selectivity for phenylphosphonic acid than the non-imprinting polymer (NIP) over its structural analog benzoic acid. The MIP was also used as a molecular receptor to recognize tyrosine phosphopeptides in aqueous media, and showed a clear preference for tyrosine phosphopeptides over interfering serine peptides compared to TiO2. These results revealed the feasibility of the use of MIPs to effectively mimic the epitope approach, and provided a promising alternative to the immunoaffinity techniques commonly used for capturing tyrosine phosphopeptides. Copyright © 2013 Elsevier B.V. All rights reserved.
Quishui Li, Feng Shen, Xiao Zhang, Yufeng Hu, Qingxi Zhang, Lin Xu, Xueqin Ren. One-pot synthesis of phenylphosphonic acid imprinted polymers for tyrosine phosphopeptides recognition in aqueous phase. Analytica chimica acta. 2013 Sep 17;795:82-7
PMID: 23998541
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