Production of 2-butanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain.

Baek-Rock Oh, Sun-Yeon Heo, Sung-Mok Lee, Won-Kyung Hong, Jang Min Park, You Ree Jung, Dae-Hyuk Kim, Jung-Hoon Sohn, Jeong-Woo Seo, Chul Ho Kim

Biotechnology letters 2014 Jan

filter terms:

Klebsiella pneumoniae was engineered to produce 2-butanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, ∆ldhA/pBR-iBO (ilvIH–ilvC–ilvD–kivd–adhA), produced 2-butanol (160 mg l−1) from crude glycerol. To increase the yield of 2-butanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of 2-butanol from 160 to 320 mg l−1. This represents the first successful attempt to produce 2-butanol from crude glycerol.

Citation

Baek-Rock Oh, Sun-Yeon Heo, Sung-Mok Lee, Won-Kyung Hong, Jang Min Park, You Ree Jung, Dae-Hyuk Kim, Jung-Hoon Sohn, Jeong-Woo Seo, Chul Ho Kim. Production of 2-butanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain. Biotechnology letters. 2014 Jan;36(1):57-62