Maki Kawai-Yamada, Minoru Nagano, Masayuki Kakimoto, Hirofumi Uchimiya
Planta 2014 JanArabidopsis cell growth defect factor-1 (Cdf1 in yeast, At5g23040) was originally isolated as a cell growth suppressor of yeast from genetic screening. To investigate the in vivo role of Cdf1 in plants, a T-DNA insertion line was analyzed. A homozygous T-DNA insertion mutant (cdf1/cdf1) was embryo lethal and showed arrested embryogenesis at the globular stage. The Cdf1 protein, when fused with green fluorescent protein, was localized to the plastid in stomatal guard cells and mesophyll cells. A promoter-β-glucuronidase assay found expression of Cdf1 in the early heart stage of embryogenesis, suggesting that Cdf1 was essential for Arabidopsis embryogenesis during the transition of the embryo from the globular to heart stage.
Maki Kawai-Yamada, Minoru Nagano, Masayuki Kakimoto, Hirofumi Uchimiya. Plastidic protein Cdf1 is essential in Arabidopsis embryogenesis. Planta. 2014 Jan;239(1):39-46
PMID: 24097264
View Full Text