Cellular localization of the atypical isoforms of protein kinase C (aPKCζ/PKMζ and aPKCλ/ι) on the neuromuscular synapse.

Núria Besalduch, Maria A Lanuza, Neus Garcia, Teresa Obis, Manel M Santafe, Marta Tomàs, Mercedes Priego, Josep Tomàs

Neuroscience letters 2013 Nov 27

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Several classic and novel protein kinase C (PKC) isoforms are selectively distributed in specific cell types of the adult neuromuscular junction (NMJ), in the neuron, glia and muscle components, and are involved in many functions, including neurotransmission. Here, we investigate the presence in this paradigmatic synapse of atypical PKCs, full-length atypical PKC zeta (aPKCζ), its separated catalytic part (PKMζ) and atypical lambda-iota PKC (aPKCλ/ι). High resolution immunohistochemistry was performed using a pan-atypical PKC antibody. Our results show moderate immunolabeling on the three cells (presynaptic motor nerve terminal, teloglial Schwann cell and postsynaptic muscle cell) suggesting the complex involvement of atypical PKCs in synaptic function. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Citation

Núria Besalduch, Maria A Lanuza, Neus Garcia, Teresa Obis, Manel M Santafe, Marta Tomàs, Mercedes Priego, Josep Tomàs. Cellular localization of the atypical isoforms of protein kinase C (aPKCζ/PKMζ and aPKCλ/ι) on the neuromuscular synapse. Neuroscience letters. 2013 Nov 27;556:166-9