Assembly factors for the membrane arm of human complex I.

Byron Andrews, Joe Carroll, Shujing Ding, Ian M Fearnley, John E Walker

Proceedings of the National Academy of Sciences of the United States of America 2013 Nov 19

filter terms:

Mitochondrial respiratory complex I is a product of both the nuclear and mitochondrial genomes. The integration of seven subunits encoded in mitochondrial DNA into the inner membrane, their association with 14 nuclear-encoded membrane subunits, the construction of the extrinsic arm from 23 additional nuclear-encoded proteins, iron-sulfur clusters, and flavin mononucleotide cofactor require the participation of assembly factors. Some are intrinsic to the complex, whereas others participate transiently. The suppression of the expression of the NDUFA11 subunit of complex I disrupted the assembly of the complex, and subcomplexes with masses of 550 and 815 kDa accumulated. Eight of the known extrinsic assembly factors plus a hydrophobic protein, C3orf1, were associated with the subcomplexes. The characteristics of C3orf1, of another assembly factor, TMEM126B, and of NDUFA11 suggest that they all participate in constructing the membrane arm of complex I.

Citation

Byron Andrews, Joe Carroll, Shujing Ding, Ian M Fearnley, John E Walker. Assembly factors for the membrane arm of human complex I. Proceedings of the National Academy of Sciences of the United States of America. 2013 Nov 19;110(47):18934-9